Journal: The Journal of Biological Chemistry

Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism

doi: 10.1016/j.jbc.2026.111137

Figure Lengend Snippet: CSPG4 is upregulated by TcdB independent of YAP . A , immunoblots from protein lysates acquired from pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. B , heatmaps depicting Z-score values for YAP regulated genes generated from RNA-seq analysis of pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. C , heatmaps showing Z-score values for MTF1 regulated genes generated from RNA-seq analysis of pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. D , immunoblots from protein lysates taken from HeLa cells transfected for 48 h with control plasmid (pCMV-GFP) or a plasmid for expressing YAP S5A (pCMV-flag YAP2 5SA). For the final 24 h, the transfected cells were exposed to 1 ng/ml of TcdB.

Article Snippet: HeLa cells were transfected with the plasmid pCMV-flag YAP2 5SA, which was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371 ) ( ).

Techniques: Western Blot, Generated, RNA Sequencing, Transfection, Control, Plasmid Preparation, Expressing